실크 피브로인 지지체와 Substance P를 이용한 골 이식재
Silk Fibroin and Substance P Combination Graft for the Reconstruction of a Bone Defect
박기유, 김성곤, 최교희, 박영주, 송지영, 조유영, 권해용, 강석우,
소속 상세정보
박기유 ( Park Ki-You ) - 한림대학교 의학과 치과학교실
김성곤 ( Kim Seong-Gon ) - 강릉원주대학교 치과대학 구강악안면외과학교실
최교희 ( Choi Kyo-Hee ) - 한림대학교 의학과 치과학교실
박영주 ( Park Young-Ju ) - 한림대학교 의학과 치과학교실
송지영 ( Song Ji-Young ) - 강릉원주대학교 치과대학 구강악안면외과학교실
조유영 ( Jo You-Young ) - 농촌진흥청
권해용 ( Kweon Hae-Yong ) - 농촌진흥청
강석우 ( Kang Seok-Woo ) - 농촌진흥청
KMID : 0360120110330040293
Abstract
PURPOSE: Substance P is a well known neurotransmitter and has been known to mediate pain. Recently, it has been unveiled that substance P is involved in the recruitment of mesenchymal stem cells to wound sites. The purpose of this study was to exam bone formation when a combination of substance P and silk fibroin was used in a bone defect model.
METHODS: Twenty rabbits were used and 40 calvarial defects were formed. They were divided as 4 groups (unfilled control, silk only, silk+10microg/ml substance P; Sub10, and silk+100microg/ml substance P; Sub100). All animals were humanely sacrificed 4 or 8 weeks after grafting. The specimens were analyzed by micro-computerized tomography and histological analysis.
RESULTS: When compared to the unfilled control to silk only group, there was significant difference in bone mineral density (BMD) and the attenuation coefficient (AC) at 4 weeks (P=0.037 and 0.038, respectively). When compared Sub10 group to Sub100 group, there was significant difference in BMD and AC at 8 weeks (P=0.004 for all). Residual graft amounts were 52.1+/-15.8%, 15.2+/-9.2% and 9.0+/-3.3% for silk only, Sub10, and Sub100 groups, respectively. When comparing the residual graft amount of silk only to sub10 or sub100, the differences were statistically significant (P<0.001).
CONCLUSION: The silk fibroin scaffold showed higher BMD and AC than the unfilled control. The combination graft with substance P and silk fibroin scaffold showed a faster graft degradation than with a silk fibroin scaffold only.
키워드
Substance P;Silk fibroin; Bone graft
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